Flow Cytometry and Sorting
نویسندگان
چکیده
منابع مشابه
Application of flow cytometry and cell sorting to megakaryocytopoiesis.
We have employed flow cytometry (FCM) and cell sorting to quantitate and study megakaryocytes in mouse and rat femoral marrow following their 20- to 30-fold concentration by centrifugal elutriation (CE). This enrichment of megakaryocytes permitted the first determination of their DNA-related fluorescence by FCM analysis following DNA staining. Fluorescence distributions of CE-enriched cell frac...
متن کاملFlow Cytometry-Based Analysis and Sorting of Lung Dendritic Cells
Dendritic cells (DC) are very specialized antigen presenting cells which play critical roles in innate and adaptive immunity and in tolerance. Their major functions are to sample antigens throughout the body and to stimulate or tolerize T cells. DC consist of several distinct subsets with different tissue distribution, surface markers, and function. This chapter discusses the lung DC subsets, t...
متن کاملUltrahigh-throughput sorting of microfluidic drops with flow cytometry.
The detection and sorting of aqueous drops is central to microfluidic workflows for high-throughput biology applications, including directed evolution, digital PCR, and antibody screening. However, high-throughput detection and sorting of drops require optical systems and microfluidic components that are complex, difficult to build, and often yield inadequate sensitivity and throughput. Here, w...
متن کاملMack Jett Fulwyler, pioneer of flow cytometry and flow sorting (1936-2001).
In any society, professional or otherwise, certain individuals stand out by virtue of their ability, leadership qualities, other personal attributes, or combinations thereof. Mack Fulwyler had all of these but somehow even more. His philosophy of life, revealed in the manner with which he dealt with others, reminded one of Henry Thoreau with respect to the grandeur of nature, Thomas Edison with...
متن کاملMeasurement and purification of human chromosomes by flow cytometry and sorting.
The 24 human chromosome types of normal diploid fibroblast cell strain were classified into 15 groups by high-resolution flow cytometry on the basis of 33258 Hoechst fluorescence. Chromosomes associated with each group were flow sorted onto microscope slides and identified by quinacrine banding analysis. DNA cytophotometry of metaphase chromosomes from the same cell strain supported and extende...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1980
ISSN: 0021-9746
DOI: 10.1136/jcp.33.6.607-d